Product description:

English name: Streptavidin from Streptomyces avidinii
Other names: chain avidin; streptavidin avidin; streptavidin
CAS: 9013-20-1
Molecular Weight: 60kD

Level: Biotechnology grade
Vitality: ≥17 units / mg protein
Dnase, Rnase & protease activity: None detected
Electrophoresis (One Band): Pass

Product description: Streptomyces avidinii A 60kD tetrameric protein that exhibits high binding affinity for biotin Able to bind one molecule of biotin with each subunit, streptavidin is useful in affinity chromatography, immunohistochemical, ELISA, and western blotting procedures using biotin-labeled antibodies and enzymes. .
Characters: white or off-white lyophilized powder derived from Streptomyces, purified by affinity chromatography by four identical peptide sequence chains, each SA can bind a peptide chain biotin molecules which in practical applications. detection sensitivity significantly better than avidin, the reason is streptavidin is a bit acidic protein, each peptide chain containing 159 amino acid residues, wherein the content of lysine, arginine and other basic amino acids than the average avidin less, whereas more acidic amino acids, their peers at 6.0, compared with avidin (pI = 10) is low, while in the structure streptavidin does not contain any sugar group, and avidin sugar up to 10% higher pI and high sugar structure avidin resulted in polystyrene plates and nitrocellulose membranes and cellular DNA or tissue binding, easy to produce non-specific binding to a certain extent, resulting in significantly higher color background, and streptavidin in the application clearly overcome this shortcoming
Use: Biochemical studies streptavidin is a tetramer protein, has superior non-covalent interactions between it and biotin, so that this small molecule - protein affinity tag to become the most practical. and most widely used system. streptavidin can bind alkaline phosphatase or peroxidase. After binding oligonucleotide probe labeled with biotin and can be carried by an enzyme and a chromogenic substrate After the material in strips (spot) position on the color reaction was screened out and can target gene (DNA fragments) Location
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