English name: Concanavalin A (jack bean); Con A
Other names: concanavalin A; Con; concanavalin A; Con A; bean protein A ball
Rating: pure affinity chromatography
Hemagglutination vitality: 1.95ug / ml (when Con A concentration of 1.95mg / ml, can avoid the occurrence of agglutination of red blood cells, red blood cells their ability to avoid aggregation may be D- glucose (D-Glc), D- mannose (D-Man), N- acetylglucosamine (N-GlcNAc), and sucrose inhibition)
Lymphocyte transformation rate:) 75%
Electrophoresis: polyacrylamide gel electrophoresis (PAGE) in PH8.9, Tris-EDTANa2-borate buffer, and displays a major protein band and a band to the protein, consistent with the standards.
A plant purified by affinity chromatography freeze-dried salt-free lyophilized powder was dissolved in water, saline or buffer solution clarity, no insolubles extract derived from beans and peas and other grains in: traits. lectin. tetrameric immunoglobulin, the molecular weight of 102000. per 237 amino acid residues containing subunit molecular weight of 25500, a combination of a Ca2 + and Mn2 +, having a carbohydrate binding site. with α- mannose, α- glucose (membrane glycoprotein) specific binding, binding site requirements are α-D- mannopyranosyl or α-D-glucopyranose six-membered ring of C-3 / C-4 and C-6 is unsubstituted hydroxy can be mixed with with α-12 glycosidic linkage mannose mannose trisaccharide and disaccharide has the greatest affinity
Use: Biochemical studies it is promoting cell mitogen (mito-gen), mainly T lymphocytes stimulate action, and animal sperm agglutination of red blood cells, inhibition of tumor cell motility and prolong allograft survival time of an important role. biochemical and immunological research reagents.
Save: 2 ~ 8 ℃