Other names : PUC19 plasmid
Characters : Powder is a commonly used cloning vectors, the transformation from pBR322 containing the ampicillin resistance gene, Lac Z gene and the same cloning sites M13mp18 PUC19 and PUC18 two plasmids structure is almost exactly the same. , but in the opposite direction of arrangement of the multiple cloning site. screening recombinant pUC18 / 19 transformed cell clones available blue / white method.
Host strain : It is recommended to use E.coli DH5α, JM109 and make the recipient strain XL1-Blue
Use: Biochemical studies cloned insert DNA fragment sequencing DNA molecular weight standards prepared. .
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