Product description:

English name: HK; Hexokinase from Saccharomyces cerevisiae; ATP: D-Hexose-6-phosphotransferase; Hexokinase from yeast
Other names: hexose kinase
CAS: 9001-51-8

Level: BR

Type: Type F-300
Molecular Weight: ~ 54 kDa (monomer); ~ 110 kDa (dimer)
Vitality: ≥130 units / mg protein (biuret)

Dynamic definition of: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 ℃
Optimal pH: 7.5 ~ 9.0

Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Anion traces: sulfate (SO42-): free

Foreign activity: ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%
. Properties: white flaky yeast extract powder catalyst (Activators): Hexokinase requires Mg2 + ions (KM = 2.6 mM) for activity Hexokinase is activated by catecholamines and related compounds; inhibitors (Inhibitors):.. Sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2 + and 4-chloromercuribenzoate)
Uses: Biochemical studies of catalytic hexose 6 on the ATP phosphorylation reaction so as to produce an enzyme of α-D-glucose high affinity (Km = 1 * 10-5M), on the other hexose also. can be phosphorylated. The reaction equilibrium shift to the right. • G ° '= 4.0 kcal. The enzyme may be Mg2, K are activated, but the reagent may be HS repressor.
Save: -20 ℃