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  • Hexokinase
  • Hexokinase
Product code: 23891700001
Unit price: 1600 CNY  (232.56 USD)
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English name: HK; Hexokinase from Saccharomyces cerevisiae; ATP: D-Hexose-6-phosphotransferase; Hexokinase from yeast
Other names: hexose kinase
CAS: 9001-51-8

Level: BR

Type: Type Ⅲ
Molecular Weight: ~ 54 kDa (monomer); ~ 110 kDa (dimer)
Vitality: ≥25units / mg protein (biuret)

Dynamic definition of: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 ℃
Optimal pH: 7.5 ~ 9.0

Protein by biuret: ≥60%

Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Foreign activity: glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%
. Characters: white powder or scaly powder yeast extract catalyst (Activators): Hexokinase requires Mg2 + ions (KM = 2.6 mM) for activity Hexokinase is activated by catecholamines and related compounds; inhibitors (Inhibitors):.. Sorbose-1- phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2 + and 4-chloromercuribenzoate)
Uses: Biochemical studies of catalytic hexose 6 on the ATP phosphorylation reaction so as to produce an enzyme of α-D-glucose high affinity (Km = 1 * 10-5M), on the other hexose also. can be phosphorylated. The reaction equilibrium shift to the right. • G ° '= 4.0 kcal. The enzyme may be Mg2, K are activated, but the reagent may be HS repressor.
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