Product description:

English name: Lysostaphin; Glycyl-glycine Endopeptidase
Other names: lysostaphin
CAS: 9011-93-2

Level: BR
Molecular weight: about 27kDa
Specific activity: ≥1200u / mg
Activity was defined: One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 ° C in a 6.0 ml reaction mixture
Assaying Methods: measured by nephelometry
Lysed S. aureus: 4 ~ 32u / ml
Lysis of Staphylococcus epidermidis: 8 ~ 64u / ml
Solubility: soluble in 50mmol / L tris-CL
Solution storage method: PH7.5 enzyme solution (PH5 ~ 8) placed at 4 ℃, the basic stability of 2-3 days
Properties: white or light yellow powder, a zinc metalloprotease, the 246 amino acids of the gene expression of a nucleic acid composition has 1486 nucleotides isoelectric point of 10.5-11.0, enzymatic activity optimum pH range. 10 to 11. mercury hydroxyl benzoic acid (PCMB), benzoyl chloride (PMSF), dithiothreitol (DTT), etc. in 1mmol / L conditions, no significant effect on the enzyme activity, Zn ions exhibit a extent of activation effect; Ba ions, Mn ions, Ca ions, Mg ions and EDTA in 1mmol / L conditions, the lysostaphin exhibit some degree of inhibition
Use: Biochemical studies specific degradation of Staphylococcus aureus and other bacterial cell wall having glycine peptide bond structure, by cleavage of its cell wall to achieve sterilization purposes.
Save: 2 ~ 8 ℃