English Name: Cellulase (Trichoderma Vride G); 14- (13: 14) -β-D-Glucan 4-glucanohydrolase
Other name: 14-β-D- glucan glucoside hydrolase
Relative molecular weight: about 58.7KD
Vitality: ≥35u / mg
Activity was defined: 1g enzyme powder (or 1ml enzyme solution) at 50 ℃, pH4.8 under the conditions, 1min hydrolysis of the substrate (paper, CMC, cotton or salicin) 1μg amount of enzyme glucose is an enzyme activity unit
Properties: pale yellow or brown powder lyophilized powder, water-soluble cellulose can function in the 14-β-D- glucoside bond pH 4.0 ~ 5.5, the best 4.8, vary depending on the substrate to use... Temperature range 40 ~ 60 ℃, the best 50 ℃. Under normal circumstances, increase the amount of weight by 0.5 births substrate can be valid, the user according to the test to determine the optimal dosage. activating agents are sodium fluoride, magnesium , cobalt chloride, cadmium, calcium phosphate, and neutral salts. inhibitors cellobiose, glucose and methyl cellulose.
Use: Biochemical studies, plant cell hybridization studies, catalyzes the hydrolysis of cellulose molecules, the resulting fiber oligosaccharides, cellobiose and glucose reactants, disaccharide accumulation will inhibit the enzymatic process capable of lysing cellulose, lichenin, barley. glucan oligosaccharides and fiber to fiber cellotriose six sugar endo -14-β-D- glycosidic linkages. It does not cleave cellobiose or p- nitrophenyl -β-D- glucoside. This You can also rely on enzymes break down xyloside serine cut key cutting complete glycosaminoglycan peptide from center (glycosaminoglycan)
Save: 2 ~ 8 ℃