Cellulase R-10

Cellulase R-10

Product description:

English Name: Cellulase Onozuke R-10
Other name: 14-β-D- glucan glucoside hydrolase
CAS: 9012-54-8

Level: BR
Relative molecular weight: about 58.7KD

Source: Green Trichoderma Trichoderma Vride
Vitality: ≥10u / mg

Activity was defined: 1g enzyme powder (or 1ml enzyme solution) at 50 ℃, pH4.8 under the conditions, 1min hydrolysis of the substrate (paper, CMC, cotton or salicin) 1μg amount of enzyme glucose is an enzyme activity unit

Properties: White or light yellow powder lyophilized powder, soluble in water can be applied to the cellulose in the 14-β-D- glucoside bond pH 4.0 ~ 5.0, the best 4.8, vary depending on the substrate temperature... the range of 40 ~ 50 ℃. Under normal circumstances, increase the amount of zero point five ten thousandths of substrate weight to valid, the user according to the test to determine the optimal dosage. activating agents are sodium, magnesium, cobalt chloride, cadmium , calcium phosphate, and neutral salts. inhibitors cellobiose, glucose and methyl cellulose.
Use: Biochemical studies, plant cell hybridization studies, catalyzes the hydrolysis of cellulose molecules, the resulting fiber oligosaccharides, cellobiose and glucose reactants, disaccharide accumulation will inhibit the enzymatic process capable of lysing cellulose, lichenin, barley. glucan oligosaccharides and fiber to fiber cellotriose six sugar endo -14-β-D- glycosidic linkages. It does not cleave cellobiose or p- nitrophenyl -β-D- glucoside. This You can also rely on enzymes break down xyloside serine cut key cutting complete glycosaminoglycan peptide from center (glycosaminoglycan)
Save: 2 ~ 8 ℃