English name: Perxidase (horseradish); HRP; POD
Other names: horseradish peroxidase; peroxidase
Vitality: RZ ~ 3, ≥250units / mg (purity value RZ = A403nm / A275nm)
Activity was defined: One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 ℃
Character: brown powder freeze, combined with a colorless enzyme protein and iron porphyrin from brown glycoprotein, HRP composed of a plurality of isozymes, isoelectric point of PH3 ~ 9, due to the optimum PH for the enzyme-catalyzed hydrogen donor varies slightly different, but mostly in PH5 so. enzyme soluble in water 58% ammonium sulfate solution and less saturation. HRP enzyme cofactor and maximum protein absorption spectrum of 403nm and 275nm, respectively, generally OD403nm / OD275nm of The ratio RZ (German Reinheit Zahl) represents purity .RZ enzyme smaller value, the more non-enzymatic protein
Uses: Biochemistry enzyme-labeled antibody is one of the main raw material, is widely used in enzyme-linked second antibody reaction is manufacturing clinical diagnostic kit for diagnostic enzyme; available enzyme preparation of glucose, triglycerides,. cholesterol and other biochemical diagnostic kits and ELISA ELISA assay of enzyme-labeled antibody.
Save: -20 ℃