English name: β-Galactosidase from Escherichia coli; GAL; β-D-Galactoside galactohydrolase; Lactase
Other names: lactase; β-D- galactosidase; enzyme lactate
Molecular weight: 100 ~ 850KDa
Dynamic: 250 ~ 600units / mg protein
Dynamic definition of: One unit causes the formation of one micromole of ONP per min at pH 7.3 at 37 ℃
Properties: white or light yellow powder, odorless, slightly sweet one can hydrolysis of lactose into glucose and galactose can generally refers to the hydrolysis of β- galactosidase enzyme galactose and glycosides in the water was... Chaos liquid (mostly dissolved), not soluble in ethanol, acetone .PH range of 5.5 ~ 9.5 (25 ℃, 20hr), the optimum pH7.0, the optimum temperature is 50 ℃. When measured live with 100mM pH7.3 phosphate powder potassium buffer solution into a transparent liquid.
Use: Biochemical studies β- galactosidase can be hydrolyzed by β-1 → 4 glycosidic linkage in the monosaccharide, oligosaccharide or glycopeptide galactose residue which is mainly used for enzyme-linked immunosorbent assay, conjugation. to the antibody as a marker. Further, β- galactosidase saccharide structural analysis can also be used, and sodium ion detector detecting lactose
Save: 2 ~ 8 ℃