RNase H

RNase H

Product description:

English name: Ribonulease H
CAS: 9050-76-4

Level: BR
Molecular Weight: 18.4kDa, monomer
Source: E. coli MRE-600 cells
Concentration: 2 ~ 5u / ul
Dynamic definition: one unit of activity is defined as 37 ℃, within 20 minutes the amount of enzyme that catalyzes the production of acid-soluble product required 1nmol
Activity assay mixture: 20mM Tris-HCL (PH7.8), 40mM KC1, 8mM MgCL2, 1mM DTT, 24uM [3H] -poly (A) poly (dT), 0.03mg / ml BSA, 4% (v / v ) glycerin
Save buffer composition: 25mM HEPES-KOH (PH8.0), 50mM KC1, 0.1mM EDTA, 1mM DTT, 0.1mg / ml BSA, 50% (v / v) glycerol
10 * reaction buffer: 20mM Tris-HCL (PH7.8), 400mM KC1, 80mM MgCL2, 10mM DTT
Quality control: Correlation tests show no endo- or exo-DNase, RNase contamination
Characters: recombinase is an RNA endonuclease which is capable of specifically hybridizing to RNA hydrolyzing the phosphodiester bond of the DNA chain, it can decompose RNA / DNA hybrid system in the enzyme can not digest single strand RNA. or double stranded DNA. Inhibitors metal chelate, SH- blocking reagent
Use: Biochemical studies before removing the second strand cDNA synthesis mRNA; RT-PCR and qRT-PCR; by oligo (dt) after hybridization (A) mRNA sequence to remove the poly;; after removal of the first-strand cDNA synthesis RNA template bits. site-specific cleavage RNA; vitro-polymer Reaction polyadenylation
Save: -20 ℃, 65 ℃ for 10 minutes as inactivated