RNase T1

RNase T1

Product description:

English name: Ribonulease T1 from Aspergillus oryzae; Rnase T1
CAS: 9026-12-4

Level: BR
Molecular Weight: 11.2kDa, monomer
E. coli cells containing the cloned gene rntA Aspergillus oryzae (Aspergillus oryzae) sources: Source
Concentration: 1000U / ul
Dynamic definition: one unit of activity refers to 37 ℃, under pH7.5 conditions, dissolve yeast RNA hydrolysis 15 minutes so that the wavelength of 260nm absorbance value increases the amount of enzyme 1.0
Activity assay mixture: 50mM Tris-HCL (PH7.5), 2mM EDTA, 3mg / ml yeast RNA
Save buffer components: 50mM Tris-HCL (PH7.4) and 50% (v / v) glycerol
Quality control: Correlation tests show no DNA endo- and exo-enzymes, protease contamination, function testing method for the purification of plasmid DNA during RNA degradation (with RNase A synergy)
Inhibitors: metal ions Mg2 +, Ca2 +, Zn2 +, Fe2 +, Cu2 + (MgCL2, inhibition 100mM concentration efficiency of about 40%; suppression efficiency when CaCL210mM ​​concentration of about 30%; Zn2 +, Fe2 + and Cu2 + inhibition at 1mM effect is very strong), single Nucleotide (2-GMP, 3-GMP, etc.), guanilyl-2-5- guanosine
Inactivation: Thermal inactivation is reversible, recommended by column purification or phenol / chloroform extraction to remove the enzyme
Characters: RNase T1 is an endonuclease, the G residues site-specific degradation of single-stranded RNA enzyme by forming a nucleoside 2 ', 3'-cyclic phosphate intermediate to cut the 3'-guanosine residue. phosphodiester bond group and adjacent nucleoside 5'-OH group between the reaction product is 3'-GMP terminal oligonucleotide. RNase T1 without metal ions play an active participation
Use: Biochemical studies DNA aspirate remove the RNA; RNase protection assays, and RNase A synergy;; excluding restructuring protein extracts of RNA; detection of G-less cassette DNA template transcribed in vitro synthesized RNA RNA sequencing. transcript levels
Save: -20 ℃