DNase Ⅰ (bovine pancreatic)

DNase Ⅰ (bovine pancreatic)

Product description:

English name: Dnase Ⅰ; Deoxyribonuclease I (Bovine pancrease); Deoxyribonucleate 5'-oligonucleotido-hydrolase
Other names: deoxy-cellular nucleases; oxygen RNA oligonucleotide 5'-hydrolase; RNase-free; DNA to polymerase; decarboxylase ribonuclease (bovine pancreatic); oxygen cellular nucleases (bovine pancreatic); DNA enzyme
CAS: 9003-98-9

Level: BR
Molecular Weight: 38KDa
Vitality: ≥300Kunitz U / mg

Activity was defined: 25 ℃, the catalytic DNA polymer solution so that the increase in absorbance at 260nm 0.001 per minute for an amount of enzyme activity unit
Loss on drying: (5.0%
Residue on ignition: (1.0%
Characters: white or off-white lyophilized powder for the double-strand specific endonuclease, can degrade double-stranded DNA become oligodeoxyribonucleotide having 5'-phosphate and 3'-OH ends when there is a manganese ion. When the presence of single-stranded DNA can also degrade soluble in water, slightly soluble in 30% ethanol and acetone enzyme reaction: DNA → dinucleotide phosphate + 5' phosphate oligonucleotide 5' The optimum pH 7.8. absorptivity (E1% 280) 11.1. activator is a divalent metal ion, can be obtained from maximal activation of magnesium (Mg2 +) + calcium (Ca2 +). Inhibitors EDTA and sodium lauryl sulfate can be plus 5mmol / L calcium (Ca2 +)
Use: Biochemical Research Molecular Biology DNA of various enzymes for degrading enzymes sugar - phosphodiester bond hydrolysis phosphate backbone of these enzymes is divided into two types:... Exonuclease and within Many endonucleases .DNAase species, some single-stranded-specific, some specific double stranded, or sequence-specific (e.g., restriction endonucleases). the restriction endonuclease has a recognition sequence for a particular base capacity, this characteristic has been used in gene manipulation techniques to construct artificial clone.
Save: -20 ℃