DNase Ⅱ (pig spleen)

DNase Ⅱ (pig spleen)

Product description:

English name: DNase II; Deoxyribonuclease Ⅱ (porcine spleen)
Other names: deoxy-cellular nucleases; oxygen RNA oligonucleotide 5'-hydrolase; RNase-free; DNA to polymerase; decarboxylase ribonuclease (porcine pancreatic); oxygen cellular nucleases (pig pancreas)
CAS: 9025-64-3
Vitality:) 300kunita u / mg
Loss on drying: (5.0%
Residue on ignition: (1.0%
Characters: white or off-white lyophilized powder for the double-strand specific endonuclease, can degrade double-stranded DNA become oligodeoxyribonucleotide having 5'-phosphate and 3'-OH ends when there is a manganese ion. When the presence of single-stranded DNA can also degrade soluble in water, slightly soluble in 30% ethanol and acetone enzyme reaction: DNA → dinucleotide phosphate + 5' phosphate oligonucleotide 5' The optimum pH 7.8. absorptivity (E1% 280) 11.1. activator is a divalent metal ion, can be obtained from maximal activation of magnesium (Mg2 +) + calcium (Ca2 +). Inhibitors EDTA and sodium lauryl sulfate can be plus 5mmol / L calcium (Ca2 +)
. Usage: all kinds of enzymes for molecular biology research of these enzymes to degrade DNA sugar - phosphodiester bond hydrolysis phosphate backbone such enzymes is divided into two: exonuclease and endonuclease .DNAase many types, and some single-stranded-specific, some specific double stranded, or sequence-specific (e.g., restriction endonucleases). the restriction endonuclease has a specific base sequence recognition capability, this a characteristic gene manipulation techniques have been used to build artificial cloning. This product is for research use
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