DNase Ⅱ (pig spleen)

DNase Ⅱ (pig spleen)

Product description:

English name: DNase II; Deoxyribonuclease Ⅱ (porcine spleen); Deoxyribonucleate 3'-oligonucleotido-hydrolase
Other names: deoxy-cellular nucleases; oxygen RNA oligonucleotide 5'-hydrolase; RNase-free; DNA to polymerase; decarboxylase ribonuclease (porcine pancreatic); oxygen cellular nucleases (pig pancreas); DNA enzyme
CAS: 9025-64-3

Level: BR
Type: Type V
Vitality: ≥1000 units / mg protein

Activity was defined: One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 ℃; [Mg2 +] = 0.83 mM
RNase: (0.1%

Form: essentially salt-free
Characters: white or off-white lyophilized powder .essentially salt-free for the double-strand specific endonuclease, can degrade double-stranded DNA has become oligodeoxynucleotide 5'-phosphate and 3'-OH ends of nucleotides. When there is a presence of divalent manganese ions, can also degrade single-stranded DNA was dissolved in water, slightly soluble in 30% ethanol and acetone enzyme reaction: DNA → dinucleotide phosphate + oligonucleotide 5' 5' optimum phosphate pH 7.8. absorption coefficient (E1% 280) 11.1. activator is a divalent metal ion, can be obtained from maximal activation of magnesium (Mg2 +) + calcium (Ca2 +). Inhibitors EDTA and dodecane base sodium can be added to 5mmol / L of calcium (Ca2 +)
Use: Biochemical Research Molecular Biology DNA of various enzymes for degrading enzymes sugar - phosphodiester bond hydrolysis phosphate backbone of these enzymes is divided into two types:... Exonuclease and within Many endonucleases .DNAase species, some single-stranded-specific, some specific double stranded, or sequence-specific (e.g., restriction endonucleases). the restriction endonuclease has a recognition sequence for a particular base capacity, this characteristic has been used in gene manipulation techniques to construct artificial clone.
Save: -20 ℃