DNase Ⅱ (pig spleen)

DNase Ⅱ (pig spleen)

Product description:

English name: DNase II; Deoxyribonuclease Ⅱ (porcine spleen); Deoxyribonucleate 3'-oligonucleotido-hydrolase
Other names: deoxy-cellular nucleases; oxygen RNA oligonucleotide 5'-hydrolase; RNase-free; DNA to polymerase; decarboxylase ribonuclease (porcine pancreatic); oxygen cellular nucleases (pig pancreas); DNA enzyme
CAS: 9025-64-3

Level: BR
Type: Type IV
Vitality: 2000 ~ 6000 Kunitz units / mg protein (biuret)
Activity is defined: One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 ° C; [Mg2 +] = 0.83 mM
RNase: (0.05%
Protein: 60 ~ 80%
Characters: white or off-white lyophilized powder for the double-strand specific endonuclease, can degrade double-stranded DNA become oligodeoxyribonucleotide having 5'-phosphate and 3'-OH ends when there is a manganese ion. When the presence of single-stranded DNA can also degrade soluble in water, slightly soluble in 30% ethanol and acetone enzyme reaction: DNA → dinucleotide phosphate + 5' phosphate oligonucleotide 5' The optimum pH 7.8. absorptivity (E1% 280) 11.1. activator is a divalent metal ion, can be obtained from maximal activation of magnesium (Mg2 +) + calcium (Ca2 +). Inhibitors EDTA and sodium lauryl sulfate can be plus 5mmol / L calcium (Ca2 +)
Use: Biochemical Research Molecular Biology DNA of various enzymes for degrading enzymes sugar - phosphodiester bond hydrolysis phosphate backbone of these enzymes is divided into two types:... Exonuclease and within Many endonucleases .DNAase species, some single-stranded-specific, some specific double stranded, or sequence-specific (e.g., restriction endonucleases). the restriction endonuclease has a recognition sequence for a particular base capacity, this characteristic has been used in gene manipulation techniques to construct artificial clone.
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