English name: DNase II; Deoxyribonuclease Ⅱ (porcine spleen); Deoxyribonucleate 3'-oligonucleotido-hydrolase
Other names: deoxy-cellular nucleases; oxygen RNA oligonucleotide 5'-hydrolase; RNase-free; DNA to polymerase; decarboxylase ribonuclease (porcine pancreatic); oxygen cellular nucleases (pig pancreas); DNA enzyme
Level: High purified by chromatography
Vitality: ≥12000u / mg
Properties: liquid, 50% glycerol solution to double-strand specific endonuclease, can degrade double-stranded DNA becomes oligodeoxyribonucleotide having a 5'-phosphate and 3'-OH ends when there is a presence of divalent manganese ions. , the single-stranded DNA can also degrade the enzyme reaction: DNA → dinucleotide phosphate + 5' oligonucleotide 5' optimum pH 7.8 phosphate absorption coefficient (E1% 280) 11.1.. activating agent is a divalent metal ion, can be obtained from maximal activation of magnesium (Mg2 +) + calcium (Ca2 +). Inhibitors EDTA and sodium lauryl sulfate can be added to 5mmol / L calcium (Ca2 +)
Use: Biochemical Research Molecular Biology DNA of various enzymes for degrading enzymes sugar - phosphodiester bond hydrolysis phosphate backbone of these enzymes is divided into two types:... Exonuclease and within Many endonucleases .DNAase species, some single-stranded-specific, some specific double stranded, or sequence-specific (e.g., restriction endonucleases). the restriction endonuclease has a recognition sequence for a particular base capacity, this characteristic has been used in gene manipulation techniques to construct artificial clone.
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