English name: Diaphorase from clostridium kluyveri; Lipoyl Dehydrogenase
Other names: diaphorase; diaphorase; dihydro-diaphorase; dihydro lipoic acid dehydrogenase
MW: 102 000 ~ 110 000
Vitality: ≥30units / mg
Activity was defined: 1 unit equals a decrease in absorbance at 600nm of 1.0 per minute at 5 ℃, pH7.5
Traits: yellow light brown crystalline or lyophilized presented fluorescent yellow-green powder, optimum pH 7.5 N- ethyl maleimide its inhibition.
Use: Biochemical studies Determination of lactate dehydrogenase in addition to a catalytic dihydro lipoic dihydro lipoic acid amide or dehydrogenation, were to become lipoic acid amide and lipoic acid, but also catalyzes the reduction of artificial electron acceptors, such as certain dyes. iron cyanate, quinone while removing two hydrogen will FAD (flavin adenine dinucleotide) is reduced to FADH2, before being passed to NAD + (nicotinamide adenine dinucleotide +) to form NADH and H +, the enzyme is mainly used for biochemical reagents
Save: 2 ~ 8 ℃, dark