English name : Proteinase K solution
Level : PCR Grade
Molecular Weight : ~ 18kDa
Vitality : ≥600U / ml, 35 ℃
Concentration : 20mg / ml
DNase, RNase: None detected
Properties: liquid, is a non-specific, subtilisin -related serine proteases derived from Candida albicans, with high specific activity .EDTA and other chelating agents or SDS and other detergents can not make a protease (Tritirachium album..) K inactivation, but enhanced activity and stability, and (PH4-12.5) are active in a wide range of PH in the tissue or cells isolated nucleic acids, nucleic acid enzyme (RNA and DNA), and the reaction of other enzymes loss live.
Uses : Biochemical studies of broad-spectrum protease, 95C heat for 10 minutes, the complete inactivation of alternative RNA extraction with DEPC-treated centrifuge tubes and pipette tips to achieve the purpose of inactivated RNase A, but also for treatment of RNase-Free water. fully degrade tissue or cell protein is a nucleic acid molecule in particular, to achieve high-quality release and extraction of nucleic acids auxiliary agents. for plasmid or genomic DNA, RNA and protein isolated polypeptide western blot .
Save : 2 ~ 8 ℃