English name: CIP; CLAP; Phosphatase, Alkaline from calf intestine
Other names: Alkaline phosphatase
Molecular Weight: The enzyme is a dimer from two 65kDa subunits size composition.
Source: calf intestinal mucosa
Concentration: 1u / ul
Dynamic definition: one unit is defined as: the amount of enzyme per minute at 37 ℃ catalyzed hydrolysis of p-nitrophenyl phosphate 1mmol 4- desired reaction conditions:. 1M diethanolamine, 10.9mM 4- nitrophenyl phosphate, 0.5mM MgCl2 (pH 9.825 ℃)
Quality control: active, endonuclease, DNase, RNase, blue / white cloning detection
1. To provide a high concentration of the product:. Cat # M2825 contains 1000u CIAP, at a concentration of 20u / ml.
2. The blue / white colony Verification: blue / white cloning detection is an enzyme used for cloning provides a high level of quality control.
3. Provide 10 * reaction buffer: 0.5M Tris-HCl (pH 9.325 ℃), 10mM MgCl21mM ZnCl210mM spermine
Storage Buffer: 10mM Tris-HCl (pH 8.0), 1mM MgCl20.1mM ZnCl250mM KCl, 50% (v / v) glycerol
Product description: calf intestinal alkaline phosphatase catalytic reaction on the DNA, RNA, ribose and deoxyribonucleoside triphosphate hydrolysis 5`- phosphate groups of the enzyme by eliminating 5`- terminal phosphate group, prevents linearization 5'-terminal phosphorylation recircularized DNA cloning vector and then connect, can be used for DNA or RNA dephosphorylation facilitate with [32P] ATP and T4 Polynucleotide Kinase (T4 polynucleotide kinase) labeled .CIAP For 5` overhang 5'female end and blunt ends are active (1-5)
Characters: suspension inhibitors metal chelators, inorganic phosphate or phosphate analog calf intestinal alkaline phosphatase catalytic DNA solutions, RNA and nucleotides 5'- and 3'-phosphate groups from. The enzyme also may dissociate phosphate groups from proteins
Usage: biochemical research cloning vector dephosphorylation, prevent the carrier from even; PCR products were sequenced before, degradation PCR mixture dNTP; T4 polynucleotide dinase mark before DNA and RNA 5'- end dephosphorylation; DNA and RNA dephosphorylation treatment; protein dephosphorylation
Save: -20 ℃