English name : CIP; CLAP; Phosphatase, Alkaline from calf intestine
Other names : Alkaline phosphatase
Level : Conjugation grade
Source: calf intestine
Vitality Specific activity: ~ 4500u / mg
Characters : The suspension, Ammonium sulfate suspension was dissolved in water and alkaline phosphatase catalyzes the dilution buffer was removed DNA, RNA, ribonucleoside triphosphates and deoxyribonucleoside triphosphates 5 ' phosphate group due to the CIP treatment. .. after the DNA fragments lack connections 5 'end phosphatase required, and therefore they can not be self- connection ( 1 ). this feature can reduce the vector background in cloning DNA
Use: Biochemical studies excision DNA, RNA, rNTPs and dNTPs 5' -terminal phosphate group; to prevent the cloning vector from cyclization connection; protein serine, threonine, tyrosine residues dephosphorylation .
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