English name : CIP; CLAP; Phosphatase, Alkaline from E.coli
Other names : Alkaline phosphatase
Level : Chromatographically purified
Source: E. coli
Vitality : 30u / mg
Description :. PH8.0 Ribonuclease (0.0002% by weight as Rnase A using a poly C assay Phosphodiesterase not detectable when assay at 0.1mg / ml with bis (p-nitrophenyl) phosphate..
Characters : The suspension, Suspension in 2.6M ammonium sulfate of alkaline phosphatase catalyzes the removal of DNA, RNA, ribonucleoside triphosphates and deoxyribonucleoside triphosphates 5 ' phosphate group due to the DNA fragment after the CIP process is missing 5 ' terminal phosphate ligase required, and therefore they can not be self- connection ( 1 ). this feature can reduce the vector background in cloning DNA
Use: Biochemical studies excision DNA, RNA, rNTPs and dNTPs 5' -terminal phosphate group; to prevent the cloning vector from cyclization connection; protein serine, threonine, tyrosine residues dephosphorylation .
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