English name: CIP; CLAP; Phosphatase, Alkaline from E.coli
Other names: alkaline phosphatase
CAS number: 9001-78-9
Source: Escherichia coli
Activity definition: One unit hydrolyzes the 1umol of p-nitrophenol phosphate per minute at 37 c, pH8.0
Description: Partially purified salt fraction. PH 8.0, Alkaline phosphatase is a broad term as sociated with non-specific phosphomonoest erase with an alkaline PH optimum.
Traits: suspension, suspension in 2.6M ammonium sulfate. Alkaline phosphatase catalyzes removing DNA, RNA, ribose, triphosphate nucleotide triphosphate deoxyribonucleoside 5 ' phosphate group. Because CIP of missing DNA fragment ligases required 5 ' phosphate ends, so they cannot connect (1). This feature can reduce the carrier when you clone DNA background
Use: biochemical studies. Removal of DNA, RNA, rNTPs dNTPs and the 5 '-end of the phosphate group; Cyclic connections to prevent cloning vectors; Protein-serine, threonine, and tyrosine residues phosphorylation