English name : CIP; CLAP; Phosphatase, Alkaline from calf intestine
Other names : Alkaline phosphatase
Level : Conjugation grade
Source: calf intestine
Vitality Specific activity: ~ 4500u / mg
Character: lyophilized powder, Freeze-dried powder, soluble in water and dilution buffer alkaline phosphatase catalyzes the removal of DNA, RNA, ribonucleoside triphosphates and deoxyribonucleoside triphosphates 5 ' phosphate group due. CIP treated DNA fragments were missing 5 'end of the desired phosphoric acid ligase, and therefore they can not be self- connection ( 1 ). this feature can reduce the vector background in cloning DNA
Use: Biochemical studies excision DNA, RNA, rNTPs and dNTPs 5' -terminal phosphate group; to prevent the cloning vector from cyclization connection; protein serine, threonine, tyrosine residues dephosphorylation .
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