English name: Elastase, pancreatic from porcine pancreas; Pancreatopeptidase E; Elastase
Other names: elastin protein enzymes; pancreatic peptidase E; pancreatic peptidase I; elasticity hydrolase; pancreatic elastase; elastase
Level: Highly chromatographically purified
Vitality: ≥8units / mg
Vitality Definition: One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 ℃
Properties: white or slightly yellow needle crystal lyophilized powder Molecular weight 25,000 (pancreas) was dissolved in water, it is a kind of decomposition of insoluble elastin characterized by a broad spectrum of proteolytic enzymes, which may be an animal pancreas extract or fermentation by microorganisms. prepared extracted and purified from animal pancreas elastase is a peptide chain endonuclease, containing 240 amino acid residues, isoelectric point PI9.5, best PH8.1 ~ 8.8, at 4 ~ 6 ℃, PH value of 5 to 10 activity may last a few days, the lyophilized powder can be stored at below 5 ℃ 6 to 12 months, the case of acid that is inactivated, can catalyze the hydrolysis of peptide bonds of other elastin peptidase or neutral amino acid formation.
Use: Biochemical studies of microbial elastase and pancreatic elastase, as having a wider hydrolysis characteristics, not only can degrade elastin and casein, gelatin, fibrin, hemoglobin, albumin and other proteins can be decomposed. is a broad-spectrum endo peptide chain enzyme. This enzyme can increase the activity of lipoprotein, has the effect of lipoprotein lipase A, can prevent the synthesis of cholesterol in the body and to promote their conversion to acid
Save: -20 ℃