Pfu enzyme

Pfu enzyme

Product description:

English name: Pfu DNA Polymerase
Other names: Pfu DNA Polymerase

Level: BR

Purity: ≥99.0%
Concentration: 5u / ul
Activity definition: in 74 ℃, 30 minutes to 10nmol of dNTPS penetrate acid insoluble precipitate the desired amount of enzyme
Product composition: Pfu DNA Polymerase; 10 * PCR Buffer
10 * PCR Buffer: 200mM Tris-HCL (PH8.8), 100mM KC1, 160mM (NH4) 2SO420mM MgSO4, 1% Triton X-100, 1mg / ml BSA
Storage buffer: 100mM Tris-HCL (PH8.3), 0.1mM EDTA, 0.1% Tween 20, 0.1% NP-40, 50% glycerol
Characters: Liquid .Pfu DNA Polymerase from high temperature thermophilic bacteria Pyrococcus furiosis separated high-fidelity DNA polymerase Pfu DNA Polymerase temperature has 5'-3 'exonuclease activity, can correct errors produced during PCR. all DNA polymerases now been found that the high temperature of the lowest error rate in a PCR process, the error rate of Taq DNA Polymerase than the average about 10 times lower. Pfu DNA Polymerase per minute extension rate of about 800 bp The optimum stretching temperature is 65-75 ℃, PCR product was blunt-end, 3 'end with no single dA
Use: Biochemical studies by testing without exonuclease activity to lambda DNA as the formwork can be a good 2kb amplified DNA fragment can be used in conventional PCR amplification, high fidelity amplification and gene expression, gene. site-directed mutagenesis
Save: -20 ℃, the shelf life of 1 year