English name: PK; Pyruvate Kinase from microorganism; ATP: pyruvate 2-O-phosphotransferase
Other names: an enzyme called pyruvate phosphate transfer; pyruvate kinase phosphorylation
Molecular Weight: 68KDa
Vitality: ≥300units / mg protein
Activity was defined: 1 U will convert one micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.2 and 30 ° C
pH stability: 5.0 ~ 10.0 (37 ℃, 20hr)
Optimum PH: 7.5
Thermal stability: ≤60 ℃ (pH8.5, 20min)
Optimum temperature: 80 ° C
Preparation instructions: The enzyme is reconstituted in 50mM Tris-HC1 buffer, pH8.5 for activity assay
Properties: White powder, composed of four subunits (molecular weight 5500) composed of long-chain fatty acids, acetyl-coenzyme A, ATP and alanine inhibit the enzyme activity, 16-diphosphate have activation.
Use: Biochemical studies pyruvate kinase involved in a step 10 the glycolytic pathway kinase reaction is an allosteric enzyme that catalyzes the conversion of phosphoenolpyruvate formed, while the phosphorylation of ADP to generate ATP..
Save: -20 ℃