English name: UAO; Uricase; Urate oxidase
Other names: acid enzymes; Urate oxidase
CAS number: 9002-12-4
Molecular weight: 34KDa (SDS-PAGE)
Activity: ¡ý 10unit/mg protein
Activity definition: in conditions of pH 8.525 ° c per minute, required for the oxidation of uric acid 1umol enzyme
Isoelectric point PI:5.4
PH stability: 7.0~11.0
Thermal stability: (50 deg c (PH8.510min)
Optimum temperature: 45 ℃
Usage: when measuring, with 0.001% Triton X-100 and 1mM the 50mM acid EDTA buffer solution (PH8.5) dissolving lyophilized
Description: from Aspergillus Niger, Aspergillus flavus, Candida yeast extract broth. White powder or grey to greenish crystalline or glassy pieces. Practically insoluble in water, and slightly soluble in alkaline buffer. Enzyme inhibition of cyanide, inhibitors such as Ag+, Hg2+.
Use: biochemical studies. Can catalyze the rapid oxidation of uric acid, the resulting allantoic acid. Used to determine the amount of uric acid in serum and urine.