English name: AP-M; LAP; Leucine Aminopeptidase, microsomal from porcine kidney; α-Aminoacyl-Peptide Hydrolase (microsomal); Amino Acid Arylamidase; Aminopeptidase M
Other names: leucine aminopeptidase; peptide chain exonuclease; leucine aminopeptidase; aminopeptidase M
Vitality: 10 ~ 40 units / mg protein (Bradford)
Activity was defined:.. One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 ° C (At 25 ° C, approx 40% of the activity at 37 ° C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate
Characters: The suspension .Suspension in 3.5 M (NH4) 42SO4 solution, pH 7.7, containing 10 mM MgCl2 is one component of the enzyme in urine, intracellular lysosomal hydrolase, is widely distributed in the kidney, liver, connective tissue. , salivary gland, lymph nodes and other tissues. molecular weight of about 255000
Uses: Biochemical studies of proteolytic enzymes aminopeptidase enzymes such enzymes from the free amino terminus of the polypeptide chain (N terminal) one by one from outside to inside the cut at the amino acid leucine aminopeptidase not Because only hydrolysis leucine (Leu) for the N-terminal residue of a peptide bond, but hydrolysis of the N-terminal leucine peptide bond have the fastest rate of origin of the name. In fact, it may act also Other amino acids in the N-terminus. Mg2 + or Mn2 + is required for the enzyme catalysis. It is commonly used in the early determination of the amino acid sequence of a polypeptide.
Save: 2 ~ 8 ℃