Phosphodiesterase I

Phosphodiesterase I

Product description:

English title : Phosphodiesterase I from Crotalus adamanteus venom; 5'-Exonuclease; Oligonucleate 5'-nucleotidohydrolase

Other names : phosphodiesterase; phosphodiesterase

CAS: 9025-82-5

Level : BR

Activity: ≥20u / mg

Activity was defined : One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25 ℃, pH 8.9.

Product description :. Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa Phosphodiesterase is. inhibited by reducing agents such as glutathione, cysteine ​​and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2 +

Appearance: white or almost white powder

Use: Biochemical research .

Save : -20 ℃